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After 48 h, biofilms were washed once with 1 PBS followed by treatment with 30 mM GSH (pH 7 0.2) and 30 mM GSH (pH 7 0.2) + 4 g/mL amikacin and also with 30 mM GSSG and 30 mM GSSG + 4 g/mL amikacin for 24 h, at 37C and 150 rpm

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This results in low vitamin B12 or deficiency and causes abnormally large red blood cells to be produced, which cannot carry oxygen around your body effectively Diet If your diet is lacking in red meat, Fish, meat, eggs, milk, and other dairy or breakfast cereals which also contain vitamin B12, you may develop vitamin B12 deficiency over time