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Subsequently, samples were sonicated to ensure complete disruption of all cells using a sonication bath (Transsonic 460, Elma Sonic, Singen, Germany) for 5 min at the highest frequency on ice

pmid:12234732 13

Jin M, Yi X, Liao W, Chen Q, Yang W, Li Y, Li S, Gao Y, Peng Q, Zhou S (2021) Advancements in stem cell-derived hepatocyte-like cell models for hepatotoxicity testing
In contrast, others found consistently lower sperm DNA fragmentation levels in the postoperative period (e.g., three months), without further significant improvement over time (61, 156)
