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Animals are randomized into treatment cohorts

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After incubation at 37 C with shaking (500 rpm) for 1 h., the cells were pelleted at 5,000 x g for 10 min at 4 C, washed once with ice-cold phosphate-buffered saline (PBS, pH 7.4), resuspended in 200 L of cold TE lysis buffer (30 mM TrisHCl, 1 mM EDTA, pH 8.0), and transferred to Lysing matrix B tubes (MP Biomedical)

Early small trials suggested that TUDCA might slow functional decline in ALS patients, leading to larger studies.38 TUDCA was later tested in combination with sodium phenylbutyrate (marketed as Relyvrio/AMX0035)