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Gambicin: a novel immune responsive antimicrobial peptide from the malaria vector Anopheles gambiae

PA14 from overnight cultures was spread on either high osmolarity PGS (pepton-glucose-sorbitol) agar plates for fast killing assays or 140% Bacto-peptone NGM agar plates for mRNA expression assays were prepared according to ref

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Notably, we observed the delipidation of VIT-2::GFP-containing particles in embryos with NR 2p-FLIM (separate GFP and Nile Red signals in the embryos shown in Supplementary Figure S5), in agreement with the previous BCARS finding ( Under low-concentration Nile Red vital staining conditions, most of the Nile Red molecules have been reported to label acidic gut granules rather than lipid particles in the intestine (Supplementary Figure S3)