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Stage 2 (N2)

2 measured reduced and oxidized hepatic GSH concentrations during alcoholic and nonalcoholic liver injury in 35 chronic alcoholics, 20 nonalcoholic patients with liver disease (chronic active hepatitis, chronic persisting hepatitis, steatosis, cirrhosis), and 15 control patients (admitted for uncomplicated abdominal procedures)

Determine the column volume required to purify the protein of interest

Olejnik, J., Hume, A