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Description
Epithelial cells from Lgr5 DTR-EGFP/+ organoids were gated as EpCAMCD45 CD31 Ter119 7AAD , and Lgr5-EGFP fluorescence intensity was used to classify cells into GFPhigh and GFPneg populations, where the GFPneg gate was defined based on the background fluorescence intensity of WT control organoids lacking EGFP expression
10.1590/s0102-865020190070000008 222 WeiY
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