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[4] US Food and Drug Administration
The FDA cited three concerns: potential immunogenicity (the peptide could trigger immune reactions), manufacturing impurity risks (synthetic peptides can contain truncated sequences and other contaminants), and a lack of human safety data sufficient to evaluate risk
For a cardiovascular performance assay, six endpoints were chosen to evaluate the cardiovascular performance in the zebrafish larvae: the heart rate and time interval in ventricles, stroke volume, cardiac output, ejection fraction, and shortening fraction
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