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Supernatants were transferred to new sets of Eppendorf tubes, desolvated in a speed vacuum concentrator, and resuspended in 20 L of buffer composed of 42.5% acetonitrile, 57.4% water, 0.1% formic acid, and 10 mM ammonium formate

Commercial kits (Pars Azmun, Tehran, Iran) and a Mindray BS-200 autoanalyzer (Guangzhou, China) were employed to assess plasma -glutamyl transpeptidase (-GT), total bilirubin, alkaline phosphatase (ALP), alanine aminotransferase (ALT), and aspartate aminotransferase (AST) [64, 66]

J Am Acad Dermatol 72(2):359361

Heat shock factor A1s are required for phytochrome-interacting factor 4-mediated thermomorphogenesis in Arabidopsis