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Description
Antioxidants & Redox Signaling, 2012
For 3-MST , CSE , UCP3 and beta-actin, RT-PCR amplification reaction was performed in a volume of 20 l containing 0.9 l Gene Expression Assay (Thermo Fisher Scientific), 10 l TaqMan Fast Universal PCR Master Mix and 2 l cDNA
doi: 10.3390/ph18020247
The 2AB that is formed when 2-oxobutyric acid accepts an amino group is abundant in the liver and is a good substrate for the -GCS reaction instead of Cys
