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After gel formation, 1 10 5 HUVECs pretreated with or without BPC 157 for 24 h were seeded into each well with 0.5 ml of M199 supplemented with 16% fetal bovine serum and 20% EGM-2

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doi: 10.1038/s41598-018-22081-7

This is one reason public health sources warn against trying to fix nitrate issues by boiling
