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For investigation of the enzymatic effect of LPL, Huh7.5 cells were incubated for 30 min at 4C with 1 g/ml LPL diluted in complete DMEM in the presence of a lipase inhibitor, tetrahydrolipstatin (THL, Calbiochem, Darmstadt, Germany) at a final concentration of 50 g/ml, before adding the virus

The role of barrier-respecting correction Effective cosmetic correction of a labial melanotic macule requires the same principles as the correction of broader lip hyperpigmentation: gentle exfoliation that respects the barrier, melanin synthesis inhibition at the enzymatic source, and barrier restoration to break the inflammatory cycle

2014 May;71(10):196175

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