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Nat Med (2001) 7:81420

Cross-linking assay of Sm TG For the BSA cross-linking assay of Sm TG at different reaction times, 40 L of the reaction buffer BD [1.25 mg/mL BSA, 50 M dithiothreitol (DTT)] and 10 L of the 1 Sm TG solution in 100 mM TrisHCl (pH 8.0) were mixed and incubated for 0, 30, 60 and 120 min at 50 C

This suggests that there is an increased utilization of vitamin C when you are having an immune response, and also that you should consistently replenish vitamin C when you have an illness

[27] Autism [edit] Heavy metal toxicity has been proposed as a hypothetical etiology of autism, and dysfunction of MT synthesis and activity may play a role in this