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126 It features an increase in pathogenic communities, such as Haemophilus and Staphylococcus , as well as Actinomyces , 211 accompanied by a decrease in the number of concomitant commensal bacteria (e.g., Prevotella and Veillonella )

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After 72 or 96 h, cells were lysed, and RNA was isolated using the RNA Nucleospin RNA extraction kit (Macherey Nagel)

Moreover, multiple studies confirm the weak to nil correlation between transcript and protein abundance (Maier et al., 2009)