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Further, a pre-clinical study of metastatic lung cancer reported that TDLNs help control metastases after the primary tumor is removed but are not required for adjuvant immunotherapy efficacy (235)

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B16-F10 (1x10 5 cells/well) resuspended in DMEM medium were seeded in 24-well plate, infected with BCG (MOI 5) and cultured at 37C in an atmosphere of 5% CO 2