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ARTICLE HIGHLIGHTS Research background The excise parietal peritoneum with the underlying superficial layer of muscle tissue, since the very early events to the huge bowel adhesion, may represent a model relevant to adhesion-related obstruction and a considerable number of emergency surgical presentation

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doi:10.1073/pnas.050909910

In the second scenario, the final yield of your elution is close to 100 mg of GST-fusion protein, so youre feeling confident that you used the right binding buffer and that your protein isnt too big