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10.1186/s40170-022-00288-7 223

Isobaric labeling of digested peptides was carried out with TMT-10Plex (Thermo Fisher Scientific) reagent followed by MCX and SPE cleanup with appropriate buffer exchanges, and offline fractionation on Shimadzu Prominence with Xbridge 150 2.1 mm column (Waters) with two-minute fractions at a flow rate of 200L/min, and peptide amounts of 15mAU-equivalent aliquots from fractions 738 were concatenated

Structure-activity relationships for withanolides as inducers of the cellular heat-shock response
Assessment of glutathione/glutathione disulphide ratio and s-glutathionylated proteins in human blood, solid tissues, and cultured cells
