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The running buffer was HBS EP+ buffer (10 mM Hepes pH 7.4, 150 mM NaCl, 3 mM EDTA and 0.05% (v/v) SurfactantP20) containing 0.1% DMSO

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Growth factors (GF), coagulation factors, adhesion molecules, cytokines, chemokines, and integrins are all stored in platelets ( Figure 1 )