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After being embedded into paraffin wax, samples were cut into 5 m thick sections and stained with haematoxylin and eosin (H&E stain)

NMN is associated with a host of beneficial health effects in animal studies, including improved glucose and mitochondrial metabolism [48]

To this end, cobalamin is considered an essential pillar among nutrients protecting mitochondrial function and neurotransmitter signaling in the neuronal circuits associated with cognitive and affective behaviors

Of the two candidates, 3,3-dimethyl-1-butanol (DMB) was thought to possess a less toxic structure, so it was advanced for further screening and testing in diet-induced TMAO studies