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Cell culture was performed in Gibco Williams Medium E, Modified (WMEM) (Cat#: 12551032, Thermo Fisher Scientific, Waltham, MA) media supplemented with L-glutamine (Cat#: G7513, Sigma Aldrich, St

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Figure 4 6.3 Complementary endocrine actions: insulin sensitization, androgen modulation, and ovulatory regulation The clinical and molecular intersections of vitamin D, MI, and melatonin converge on three tightly connected endocrine domains central to the pathophysiology of PCOS: IR, androgen excess, and ovulatory dysfunction