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Generation and quantification of protein persulfides and polysulfides in cell lysates HEK 293 cell lysate (200 g) protein/sample in 50 mM HEPES buffer (pH 7.4) was treated with Na 2 S (040 M) or Na 2 S 2 (0600 M) for 7 min in sealed Eppendorf tubes with minimal head space at RT

Indeed, the normal isotope distribution of the mBBr-thiosulfate detected in the control and C408S samples (Fig

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Elevated levels of GST are associated with increased resistance to apoptosis initiated by a variety of stimuli (Kodym et al ., 1999