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The nine tissue systems now documented in the preclinical literature were not predicted by computational models or receptor-binding assays at the outset they were identified sequentially as researchers in different laboratories independently tested the compound against different experimental injury models and consistently found meaningful effects

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This led some authors to propose a chaperone competition model, in which aggregates, client, and metastable proteins (polyQ, huntingtin, ATXN1, and superoxide dismutase-1) rival for finite chaperone resources [64]