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The brain needs lots of oxygen to function optimally, which means it produces a lot of free radicals

Cell viability assay For cell viability assays, FASN +/+ -PyMT MEFs and HC11 cells were seeded (5 10 3 cells/well) in 96-well plates for 24 h and subsequently replaced with fresh medium with vehicle (control) or increasing concentration of sodium citrate (Sigma) (1, 2.5, 5.0, and 10 mM) or SB204990 (Tocris Bioscience) (10, 20, 30, 40, 60, and 100 M)

& Andrews, S

Furthermore, in diabetic kidney disease (DKD), TST deficiency contributes to the disruption of fatty acid oxidation (FAO), a critical process for energy production in renal tubular cells [117,126]