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The next day, 10% FBS containing RPMI 1640 medium was added, and cells were treated with 10 ng/ml TNF-, with and without NR 250 M, and incubated for 24 h
Given that HIF-2 is an important transcription factor in cancer progression and leads to poor prognosis 69,70 , these findings suggest that targeting HIF-2 might be an effective therapeutic strategy by inhibiting glutamine metabolism in these notorious cancers
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