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For electron microscopy examination, liver tissues were prefixed after harvesting immediately with 1.5% glutaraldehyde and 0.8% paraformaldehyde (0.1 mol/L cacodylate buffer) at room temperature and postfixed in an aqueous solution of 1% OsO 4 and 1.5% K 4 (FeCN) 6

Furthermore, RSL3-exo promotes the immune response by synergistically increasing ROS production, ferroptosis-related protein expression, and ultimately, elimination of the immunosuppressive tumor microenvironment [49]

doi: 10.1016/j.carbpol.2015.02.068 58 ZacariasM.Barn-SevillaB.LazoJ

This keto meal gets bonus points for using just one dish for easy and fast cleanup
